Infectious Diseases Diagnosis & Treatment (ISSN: 2577-1515)

case report

S. Gallinarum from Human Sample-First Case Report in India and an Alarm to Non-Vegetarians and Poultry Workers

1Consultant Microbiologist, MIT Super speciality hospital Aurangabad, Maharashtra, India

2Medical director & Intensivist, MIT Super speciality hospital Aurangabad, Maharashtra, India

3Dhule, Maharashtra India

*Corresponding Author: Karan Ostwal, Consultant Microbiologist, MIT Super speciality hospital, Aurangabad, Maharashtra, India, Tel: +91 8862079170; E-mail: karanost55@gmail.com

Received: 7 January, 2017; Accepted Date: 10 February, 2017; Published Date: 17 February, 2017

Abstract

Salmonella Enterica, Serovar Gallinarum is the causative agent of Fowl Typhoid (FT). It is an acute septicaemic disease of chickens and other galliformae birds. FT is associated with infected poultry eggs and this is the reason for transmission of FT from one generation to other. No case report of Salmonella enteritica serovar gallinarum has been reported from humans. We hereby present with the 1st case report of S.Gallinarum in a patient with severe loose motions. The isolate was confirmed using Vitek 2 system using GN ID cards. The patient recovered completely after treatment.


Keywords: Birds; Chickens; Galliformae, S.Gallinarum, Vitek 2

1. Introduction

Fowl typhoid (FT) the causative agent of an acute septicemic disease of chickens and other galliforme birds is caused by Salmonella enterica Serovar Gallinarum (SG) [1] It is one of the most important cause of poultry disease in birds and is responsible for high mortality and morbidity [2]. S.Gallinarum infection in India became prominent for the first time when it was recorded as the commonest Salmonella of avian origin at the National Salmonella Centre at Indian Veterinary Research Institute, Izatnagar [3]. 95 isolates of S. Enterica belonging to S.Gallinarum, S. Enteritidis, S. Typhimurium, S. Bareilly and S. Paratyphi B has been reported with the overall prevalence of 14.40% from North eastern region of India [4]. In the studies done in Karnataka, Maharashtra, and Tamil Nadu, the most predominant serotype isolated from poultry was S.Gallinarum [5]. Several factors can affect Salmonella colonization in poultry, including the age and genetic susceptibility of the birds, bird stress due to overcrowding or underlying illness, level of pathogen exposure (infectious dose), competition with gut microflora for colonization sites, infecting Salmonella serovar, and whether the strains carry genetic factors that facilitate attachment to the birds’ gastrointestinal tracts or evade host defenses [6]. SPI-13 and SPI-14 genes are important in the pathogenesis of S.Gallinarum [7]. The antimicrobial drugs such as β-lactams, aminoglycosides and fluoro quinolones are used to treat local and systemic infections caused by S. Gallinarum and S. Pullorum in commercial chickens [8]. Multi drug resistant S.Gallinarum is rising amongst poultry. Human salmonellas is typically associated with the consumption of contaminated foods, such as fresh and processed meat and poultry, eggs [9]. But to the best of our knowledge, no case report of S.Gallinarum causing human infection has been reported so far. We hereby report the 1st case report of S.Gallinarum from human sample.

2. Case Report

 A 40 year old farmer was admitted to the MIT super speciality hospital, Aurangabad (Maharashtra) on 13th September 2016 with complaints of fever, abdominal discomfort, loose motions up to 10 episodes per day since 4-5 days. Patient also had 3-4 episodes of vomiting since 2 days. He had history of eating non vegetarian food at a restaurant. On physical examination he appeared to be ill and toxic. He was febrile with temperature of 102ºF, blood pressure 90/60 mm Hg, pulse rate 118/min and respiratory rate 16/min. Stool sample was sent to laboratory for culture.

3. Microbiological Examination

On gross examination, the stool was liquid with no worm segments. Microscopy showed the presence of pus cells and RBC’s. Sample was inoculated on Blood Agar and MacConkey’s agar and was incubated at 37ºC for 24 hours. On blood agar and MacConkey’s agar 2 types of colonies were seen.

3.1. Colonies on Blood Agar

Colony 1: 2-3mm, greyish white, smooth, convex, opaque, beta haemolytic colonies

Colony 2:  2-3mm, circular, low convex, smooth, translucent, non haemolytic colonies

3.2. Mac Conkey’s Agar

Colony1- Lactose Fermenting, 2-3mm, circular, convex colonies.

Colony 2- Non Lactose Fermenting (NLF), circular, low convex colonies.

Both the colonies were subjected to biochemical reactions and Antibiotic Susceptibility testing. Colony 1 was identified as E.coli.

The biochemical reactions match with Salmonella and as dulcitol was utilised it raised doubt of S.Gallinarum [10]. So, they were subjected to identification by Vitek 2 compact automated system marketed by Biomerioux (France) using GN ID cards and antibiotic susceptibility was done using Kirby-Bauer disc diffusion method using discs of standard concentrations provided by Hi-media laboratory (Mumbai, India). He was found sensitive to Amoxicillin-clavulanic acid (20 mm), Ceftriaxone (25 mm), Tetracycline (18 mm), Imepenem ( 25 mm), Aztreonam (24 mm) and resistant to Amikacin (8 mm) and Norfloxacin (11 mm) [11]. He was given ceftriaxone intravenously 1gm BD for 7 days. The patient recovered completely after treatment.

4. Discussion

Over years, human infection and food poisoning by Salmonella is on rise dramatically in Europe, USA and other parts of the world. Poultry and poultry products are the major source of infection [12]. S.Gallinarum, the causative agent of FT, is the most prevalent host adapted Salmonella strain of poultry in India [13]. S.Gallinarum has increased ability of to colonize and/or survive due to an adaptive immunity in poultry and hence it is found abundantly than other Salmonella [14]. In one study, in broiler chickens out of 23 Salmonella isolates, 19 samples were identified as S.Gallinarum and 4 samples as Salmonella Enteritidis [15]. In the study made by Rajgopal et al, all the 3 isolates were S.gallinarum [16]. So it is beyond doubt that S.Gallinarum is the most prevalent Salmonella strain in poultry. But no case report has been reported from humans. Our patient had history of eating non vegetarian food 1 day back at Dhaba. So there are chances that he might have got infection from the improperly cooked food. The patient was found sensitive to most of the antibiotic except amikacin and Norfloxacin.

 This is the first case report of S.Gallinarum from human sample. Our identification was possible due to Vitek 2 system. It is difficult to identify S. Gallinarum biochemically and many sugars are required for its identification. So, this might be the possibility of non reporting of the cases till date.

5. Conclusion

We presented the first case report of S.Gallinarum from human sample. This case report might be an alarm to specially non vegetarians and poultry workers who are constantly in contact with the microorganisms. As drug resistance of S.Gallinarum amongst poultry is being on rise, the drug resistance strains might emerge in humans.


Test

Result

Motility

Non motile

Catalase

Positive

Oxidase

Negative

Indole

Negative

Methyl Red

Positive

Voges Proskauer

Negative

Citrate

Was not utilised

Urea

Not hydrolysed

Triple Sugar Iron

Alkali/acid with H2S without gas

Lysine Decarboxylation

Positive

Ornithine Decarboxylation

Positive

Nitrate

Was reduced to nitrite

Phenylalanine Deamination

Negative

Malonate Utilization

Negative

Lactose

Not fermented

Dulcitol

Fermented

Sucrose

Not fermented

Salicine

Not fermented

Inositol

Not fermented

Maltose

Fermented

Arabinose

Not Fermented

Sorbitol

Fermented

Table 1: Biochemical reactions of colony 2 (NLF Colony).

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