"Synergistic antibacterial effect of silver nanoparticles combined with ineffective beta lactam antibiotic on ESBL producing uropathogenic E.coli strains"

Bushra Uzair 1, Anum Shokat 1, Muhammad Ismail 2 and Qaiser Mansoor 2

1International Islamic University, Islamabad, Pakistan

2Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan

DOI: 10.29011/2574-7258-C1-003

The increase in antibiotic resistance among Extended spectrum beta lactamases (ESBLs) producing uropathogenic E. coli is a major concern worldwide. ESBLs are diverse group of plasmid mediated enzymes produced by Gram negative bacteria. The current study included 120 uropathogic E. coli strains isolated from a tertiary care hospital, Islamabad. A total of 72(60%) isolates were found to be ESBL producer. High rate of resistance was observed against ceftriaxone (100%), ciprofloxacin (94%) and cotrimoxazole (79%). Out of 72 ESBL E. coli isolates 51 (71%) of the isolates exhibited blaCTX-M genes, 21(29%) isolates were positive for blaSHV gene, 21(29%) isolates harbored both blaCTX-M and blaSHV genes while blaTEM gene was not detected in any isolate. Positive synergism was observed when silver nanoparticles synthesized from chemical reduction method using sodium borohydride as a reducing agent was combined with beta lactam antibiotics ceftriaxone were tested against ESBL E. coli strains. However, negative synergism was observed when silver nanoparticles and beta lactam antibiotic imipenem was combined. The present study concludes that there has been a rise in ESBL E. coli with polymorphism of blaCTX-M, blaSHV causing Urinary tract infections. The drug-AgNPs synergistic effect can be used to combat uropathogenic infections.

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