## "Association between Optical Signal Derived Aortic Augmentation Index and Cardiovascular Risk Factors in Healthy Volunteers"

### Marika Pikta1,3*, Margus Viigimaa2,3, Kristjan Pilt3, Kristina Kööts3, Kalju Meigas3

1Laboratory, North Estonia Medical Centre, Tallinn, Estonia

2Centre of Cardiology, North Estonia Medical Centre, Tallinn, Estonia

3Department of Health Technologies, Tallinn University of Technology, Tallinn, Estonia

*Corresponding author: Marika Pikta, Department of Health Technologies, Tallinn University of Technology, Tallinn, Estonia

Received Date: 05 March, 2020; Accepted Date: 19 March, 2020; Published Date: 24 March, 2020

#### Abstract

Background: The aim of this study was to determine whether the photoplethysmographic signal derived Aortic Augmentation Index (PPGAIxao) can be used as an alternative method for cardiovascular risk estimation. The relationship between PPGAIxao and the traditional cardiovascular risk factors were investigated on apparently healthy subjects.

Methods: The Photoplethysmographic (PPG) signal was registered from right hand index finger of healthy volunteers. The Arteriograph device was used as a reference for the pulse wave analysis parameters and aortic pulse wave velocity measurements. The HDL-cholesterol, LDL-cholesterol, total cholesterol and triglycerides from collected peripheral venous blood specimens were measured. The relationships between the results of arterial stiffness related parameters and the traditional cardiovascular risk factors were analyzed.

Results: Our data showed the strong correlation between Arteriograph and PPG signal derived augmentation indices (r=0.78). The PPGAIxao values were averagely 7% higher than Arteriograph estimated aortic Augmentation Index (AIxao). The PPGAIxao and AIxao showed positive correlation or did not correlate with other cardiovascular risk parameters. Both derived augmentation indices were higher in case of higher SCORE risk chart values and in case of subject groups with higher age.

Conclusion: It can be assumed that the optically estimated PPGAIxao could be considered in the future as one method for augmentation index and cardiovascular risk estimation. Future studies have to be carried out on actual patients, to improve the PPG waveform registration method.

#### Keywords

Arterial Stiffness; Augmentation Index; Cardiovascular Risk Factors; Photoplethysmography; Pulse Wave Analysis; Pulse Wave Velocity

#### Introduction

It is known that mortality from Cardiovascular Diseases (CVD) is a leading cause of mortality in all developed countries, including Estonia. In this regard, one of the priorities of healthcare is early detection of atherosclerotic vascular lesions and giving adequate treatment to patients at high risk of cardiovascular complications. Prevention and treatment of the circulatory system diseases is an important task to both healthcare and the society as a whole.

Systemic Coronary Evaluation (SCORE) risk chart for screening fatal cardiovascular complications, recommended by the ESC experts, has a limited set of variables, and does not include the parameters that characterize the vascular wall [1]. The consensus document that unifies the opinions of European experts on using arterial stiffness parameters for diagnosis and treatment (published at the end of 2006) states that measuring arterial stiffness related parameters has significant advantages over the evaluation of classical risk factors, as it directly reflects the factual damage of the vascular wall [2]. Currently, it has been proven that the stiffness of the major arteries determined by Pulse Wave Velocity (PWV) is an independent predictor of cardiovascular mortality, fatal and nonfatal coronary events in patients with arterial hypertension or Type 2 diabetes, as well as elderly patients and total population as a whole [3-5].

Augmentation Index (AIx) is considered to be a measure of systemic arterial stiffness and is calculated from the central and radial pressure waveforms. Aortic Augmentation Index (AIxao) is related to the mechanical properties of the artery through changes in PWV. In case of elastic arteries, the reflected waves from the peripheral sites arrive back to the aortic root during diastole part of the heart cycle. Increased arterial stiffness raises the PWV and causes the early arrival (during systole part of the heart cycle) of reflected waves. This phenomenon augments the systolic and pulse pressure in aorta, increases wall stress, raises risk of atherosclerosis development and elevates left ventricular afterload [6]. However, in addition to the stiffness of the arteries, the AIxao is influenced as well by the characteristics of the left ventricle ejection profile and amplitude of the reflected waves [7,8]. An increased AIx have been associated with increased cardiovascular risk [9,10]. In addition, in the study by Schram, et al. [11] an increased AIx was reported in patients with type 2 diabetes or impaired glucose tolerance. Furthermore, AIxao has been shown to have independent predictive values for cardiovascular mortality in patients with end-stage renal disease [12]. AIx has been in focus for mechanistic analyses in therapeutics, pharmacology and pathophysiology, nevertheless more investigations are needed before it can be recommended to introduce it into routine clinical use [13].

From the scientific community and industry, there has been much interest in developing non-invasive methods and devices for cardiovascular system status evaluation [2]. SphygmoCor and Complior devices are considered as “Gold standard” for the measurement of aortic pulse wave velocity and central blood pressure. SphygmoCor enables to estimate AIxao, which is used as an indicator of aortic stiffness [14]. Aortic PWV, AIxao and central blood pressure can also be obtained Arteriograph device that has been validated with invasive methods [15]. In earlier study, three devices used to measure PWV were compared [16]. It was found that the differences were primarily caused by the differences in measuring traveled distance of the pulse wave. In current study, the Arteriograph device is used to estimate PWV and AIxao.

Previous studies have shown that Photoplethysmographic (PPG) method, which is an optical method, can offer an alternative approach for the estimation of AIxao [17]. PPG method registers the blood volume and velocity changes in the examined tissue using mainly red or infrared light. Registered light intensity changes are related to the pulse wave, which is propagating in the arterial tree [18]. The aim of this study was to determine whether the PPG signal derived aortic augmentation index (PPGAIxao) can be used as an alternative method for cardiovascular risk estimation. Therefore, the relationship between PPGAIxao and the traditional cardiovascular risk factors were investigated on apparently healthy subjects. The Arteriograph device was used as a reference for the pulse wave analysis parameters and aortic PWV measurements.

#### Study Population

The present study included 54 asymptomatic volunteers (29 men and 25 women) without known heart and vascular diseases. All subjects in this study were asked to provide their written consent. The subjects were divided into three groups on the basis of their age: 1 (age: 21 - 39 years, n=20), 2 (age: 40 - 53 years, n=25), 3 (age: 56 - 73 years, n=9). The study was carried out in North Estonia Medical Centre in collaboration with the Department of Biomedical Engineering, Tallinn Technical University. This study was approved by the Tallinn Ethics Committee on Medical Research.

#### Cardiovascular Risk Factors

Information on demographic and anthropometric characteristics and cardiovascular risk factors was collected on the day when the measurements with the non-invasive devices were carried out. We administered a structured questionnaire to obtain information on each subject: age, body mass index (was calculated), smoking status (yes/no), use of medications (had not received treatment with statins), and medical history. The WHO classification of Body Mass Index (BMI) was used to classify the patients as underweight (BMI < 18.5 kg/m2); normal (BMI 18.5 - 24.9 kg/m2); overweight (BMI 25.0 - 29.9 kg/m2); and obese (BMI > 30 kg/m2) [19]. Results of laboratory tests were recorded.

#### Laboratory Methods

All parameters were analyzed during the same day using standard methodology in an accredited laboratory. Peripheral venous blood specimens were collected, centrifuged, and biochemical measurements such as HDL-cholesterol, LDLcholesterol, total cholesterol and triglycerides were completed by Cobas-600-c501 (Roche Diagnostics GmbH, Manheim, Germany) using commercial kits.

#### Arteriograph Parameters

The central Systolic Blood Pressure (SBPao), Central Pulse Pressure (PPao), Aortic Augmentation Index (AIxao), and aortic Pulse Wave Velocity (PWVao) were estimated using an Arteriograph device (TensioMed, Budapest, Hungary) [20]. The distance between jugulum (sternal notch) and symphysis (pubic symphysis), two characteristic anatomical points, was measured in a straight line and it was ensured that the tapeline was not following the curves of the body. Second, the Arteriograph measurement was carried out. In all measurements the cuff size was set in accordance with the manufacturer’s recommendation and placed tightly around the left upper-arm. Third, the measurement with the Arteriograph was carried out. Initially, the device measured the blood pressure using an oscillometric method. Next, the pressure waveform measurements were performed when the cuff pressure exceeded systolic blood pressure by 35mmHg, with a completely occluded brachial artery. The pressure wave signal was recorded for 10 seconds. The quality of the signal was carefully examined after each measurement and if needed, the measurement was repeated.

The SBPao, PPao, AIxao and PWVao were calculated automatically by the TensioClinic version 1.10.1.1 (TensioMed, Budapest, Hungary) software. The Arteriograph calculates the central SBPao and PPao on the basis of the brachial systolic blood pressure (SBPbrach) and the recorded pressure waveform. The brachial augmentation index (AIxbrach) was calculated from the recorded pressure waveform. The AIxao was calculated using the linear model between AIxbrach and AIxao [15]. The transit time is the return time (S35) calculated by the Arteriograph from the recorded pressure waveform, which is the difference in milliseconds between the initial and the reflected systolic waves. The PWVao was calculated as the ratio between the distance of jugulumsymphysis and the return time. As the pulse wave travelled to the bifurcation and back, the distance was multiplied by two for PWVao calculation.

#### Experiment Setup and Measurements

The measured and calculated parameters and used methods are summarized in Table 1. PPG signal was registered using experimental measurement complex [21]. The PPG signal was registered from an index finger of right hand using Envitec F-322212 finger clip sensor (Honeywell, Germany). The infrared LED (880nm) of the sensor was used. The sensor was connected to the lab built module, where it was possible to set the current of the LED and gain of the signal. The LED worked in pulsed mode. The lab built module was connected to the data acquisition card PCI MIO-16-E1 (National Instruments, USA), where the analogue signal was digitized with sampling frequency of 1kHz. The PPG signal was monitored and recorded during the experiment, using the LabVIEW (National Instruments, USA) environment developed program.

All the experiments were carried out by trained personnel in a quiet room with constant temperature (23 ± 1°C). Firstly, the subject was asked to be in supine position. The PPG sensor was attached to the index finger of the subject and the current of the LED and gain of the signal were set in order to achieve the quality of the PPG signal. The maximum current of the LED in pulsed mode was 47mA. It was ensured that the LED of the sensor would not cause any heating effect to the finger. After at least 20 minutes, the measurements with Arteriograph were carried out and the parameters were calculated as previously explained. The supine position remained constant also during the one-minute-long PPG signal registration.

#### Data Analysis and Statistical Methods

The PPG signal analysis was carried out using MATLAB (The MathWorks, USA). For each subject, the periods of the PPG signal were filtered and normalized in length according to our previous study [17]. From the second derivative PPG signal the distinct peaks ‘b’ and ‘d’ were detected and the PPG signal amplitudes at these locations were measured (Figure 1). The PPGAI index was calculated according to the following equation:

where Ad and Ab are the amplitudes of the normalized PPG signal at the locations of distinctive peaks ‘d’ and ‘b’ of second PPG derivative signal, respectively. The aortic augmentation index (PPGAIxao), given in percentages, was calculated from the PPGAI index using in the previous study [17] developed model:

Statistical analysis was carried out with IBM SPSS statistics version 20. Data normality was analyzed using the Shapiro-Wilk test. The results are expressed as mean ± standard deviation or as median (25th-75th percentile) for skewed data. The difference between variables was tested using the Mann-Whitney test. Spearmans correlation coefficients were calculated to test the association between arterial stiffness related parameters and risk factors. Statistical significance was considered if p<0.05.

#### Characteristics of Study Subjects and Assessment of Risk Factors

Subjects age was between 21 and 73 years, with mean ± SD age being 43 ± 13. The subjects were divided into three groups on the basis of their age: 1 (21 - 39 years), 2 (40 - 53 years), 3 (56 - 73 years). The mean BMI was 23.5 ± 3.2kg/m2, 25.9 ± 3.4kg/m2, 24.9 ± 2kg/m2. There was no significant difference in waist circumference between groups. The levels of total cholesterol, LDL cholesterol were lower in group-1 than in group-2 and group-3, which was statistically significant (p<0.05). The peripheral systolic blood pressure was lower in group-1 than in group-2 and group-3, which was statistically significant (p<0.05). The main characteristics of the study subjects are shown in Table 2.

#### Arterial Stiffness Related Parameters

Table 3 and Figure 2 summarize the results of the aortic stiffness related parameters. The PWVao was, on average, lower in group-1 (6.8m/s (varied between 6.3m/s and 7.3m/s)) than in group-2 (7.0m/s (varied between 6.6m/s and 8.6m/s)) and group-3 (9.8m/s (varied between 8.3m/s and 12.1m/s)). The differences between the groups were statistically significant (p<0.05). The SBPao, PPao and AIxao were higher in group-3 than in group-1 (p<0.05).

The AIxao and PPGAIxao were higher for females than males (AIxao: 24% vs 14%, p=0.07 and PPGAIxao: 20% vs 9%, p<0.01, Figure 3).

#### Arterial Stiffness Related Parameters and Cardiovascular Risk Factors

PPGAIxao positively correlated with age (r=0.54, p<0.01) and has low correlation with systolic blood pressure (r=0.32, p<0.05), HDL-cholesterol (r=0.16, p=0.25), and LDL-cholesterol (r=0.13, p=0.36). Similarly, AIxao positively correlated with age (r=0.55, p<0.01) and again has low correlation with systolic blood pressure (r=0.27, p<0.01), HDL-cholesterol (r=0.28, p<0.05), or LDL-cholesterol (r=0.30, p=0.05). Likewise, PWVao also positively correlated with age (r=0.55, p<0.01) and has low correlation with systolic blood pressure (r=0.36, p<0.01), HDLcholesterol (r=0.24, p<0.05) or LDL-cholesterol (r=0.31, p<0.05). Triglyceride level did not correlate with any of the arterial stiffness related parameters.

On the basis of the SCORE risk chart, all subjects were classified into risk levels (Table 4 and Figure 4). Positive correlation was revealed between SCORE and AIxao (r=0.51, p<0.001), however weak positive correlation was found between PPGAIxao (r=0.39, p<0.001) and SCORE.

Increased SBPbrach > 135mmHg was found in 19% of subjects. Subjects with increased SBPbrach had higher PPGAIxao (20% vs 15%) and AIxao (28% vs 21%) than subjects with SBPbrach < 135mmHg. Positive correlation was found between PPGAIxao and Arteriograph estimated AIxao (r=0.78, p<0.01), which is illustrated with Bland-Altman plot in Figure 5.

#### Discussion

In this study, the aortic AIx was estimated using the noninvasive optical technique for pulse wave registration from index finger and new offline waveform analysis algorithm. The Arteriograph device was used as a reference for the pulse wave analysis parameters and PWV measurements. The main objective was to investigate the relationship between PPGAIxao and the traditional cardiovascular risk factors in apparently healthy subjects.

Positive correlation (r=0.78) was found between PPGAIxao and AIxao. The results are similar to our previous study [17], where strong correlation was found between PPGAIxao and SphygmoCor derived AIxao (r=0.85). In line with a previous study [22], our results showed that the augmentation index was higher for women.

According to the Bland-Altman plot (Figure 5b), the PPGAIxao values are on average higher (7%) than Arteriograph estimated AIxao values. Outliers can be found as well, which cause the high standard deviation in Bland-Altman plot. The differences can be explained by various factors – the measurements are not carried out simultaneously, the source of the signal differs in the physical (different type of sensor) and physiological point of view (different locations in arterial tree).

The PPG signal is optical signal, which represents the blood volume changes in the microvascular bed of tissue. The Arteriograph and SphygmoCor devices register the signal, related to the pressure wave. The volume-pressure relationship is nonlinear and the volume wave depends on the transmural pressure, which is the difference between the internal pressure and any additional externally applied pressure of the blood vessel [23]. Therefore, the PPG signal amplitude and the waveform [24] depend on the applied pressure by the sensor. In this study, all the PPG signal registrations were carried out with the same sensor. However, the blood pressure, as well as the circumference of the finger varied, which caused the change in the transmural pressure. It can be debated that the influence is minimal, nevertheless, in the future studies this effect should be taken into account and the influence on estimated PPGAIxao should be investigated.

Positive correlation of SCORE values with AIxao and weak positive correlation between SCORE values and PPGAIxao were found, according to Figure 4 the average PPGAIxao and AIxao tend to increase with the SCORE values. Due to the small number of the subjects in the “High risk” group the statistical differences between the groups were not possible to investigate. As the population in Estonia is relatively small (about 1.3 million people), it is very difficult to find elderly and at the same time healthy subjects. In addition, in Table 4 does not include “Very high risk” (>10%) group according to the SCORE risk chart, because subjects were apparently healthy and no one fit into this category. SCORE is the European cardiovascular disease risk assessment model, which has several cardiovascular risk factors (gender, age, total cholesterol, systolic blood pressure and smoking status) as input parameters. The positive tendency in the increase of PPGAIxao and AIxao together with SCORE values indicates an increased risk of cardiovascular events with a possible increase in arterial stiffness. It is also supported by the study done by Rhee, et al. [14], where the alterations in AIxao and PWVao are associated with the structural changes of atherosclerosis. It may be suggested that the vascular wall is a target organ for exposure to risk factors mentioned at the SCORE scale. It is notable that compared to the previous study [25], the PPGAIxao and AIxao showed weak positive correlation with the systolic blood pressure, HDL- and LDL-cholesterol. In addition, PWVao showed positive correlation with age, but weak associations were found with systolic blood pressure, HDLcholesterol and LDL-cholesterol, which is similar to the PPGAIxao and AIxao.

Moreover, this study did not show any association between triglyceride level and increase in parameters associated to the arterial stiffness. However, the relation between triglycerides and arterial stiffness is controversial. Our findings differ from those of Sliem, et al. [26], which demonstrated that aortic stiffness is in direct association only with triglycerides, when the components of the lipids are considered separately. Another study [27] in Greece has found that serum triglyceride levels are associated with indices of arterial stiffness. Our findings are in agreement with a study by Razman, et al. [28], which found no significant association between triglyceride level and AIxao.

The results from our study suggest that PPGAIxao, AIxao, PPao, PWVao, and SBPao, are lower in young adults as compared to elderly subjects. Our findings are in close agreement with a previous study that reported a positive correlation between increased arterial stiffness and age and systolic blood pressure [29].

#### Conclusions

According to the results, the PPGAIxao index, derived from PPG registered pulse waveform, correlates significantly with Arteriograph estimated AIxao in case of healthy subjects. The PPGAIxao values were averagely higher compared to the AIxao values. In addition, PPGAIxao and AIxao correlate only little or not at all with other cardiovascular risk parameters. Furthermore, the PPGAIxao values are higher, similarly to AIxao, for subjects with higher SCORE values and groups with higher age. Therefore, it can be assumed that the optically estimated PPGAIxao could be considered in the future as one method for augmentation index and cardiovascular risk estimation. However, more studies are needed to carry out on patients and as well to improve the PPG waveform registration method.

The authors have no conflicts of interest related to this study.

#### Acknowledgment

The research was funded partly by Estonian Ministry of Education and Research under institutional research financing IUT 19-2 and the European Union through the European Regional Development Fund. We are grateful to all of the laboratory staff and volunteers.

Figure 1: One period long PPG signal, which is filtered and normalized in length (upper graph) and the amplitudes Ab and Ad are detected at the locations of corresponding second derivative PPG signal peaks of ‘b’ and ‘d’ (lower graph).

Figure 2: Differences of AIxao and PPGAIxao for all study groups. Median (bold line), upper quartile (upper edge of gray box), lower quartile (lower edge of gray box), maximum (upper bar), minimum (lower bar) and outlier (circle) values of AIxao (%) and PPGAIxao (%) are given.

Figure 3: Variation of PPGAIxao and AIxao by gender.

Figure 4: Average values with standard deviations of PPGAIxao and AIxao according to SCORE.

Figure 5: a) The relation between the PPGAIxao and the AIxao with the regression line and the correlation coefficient. b) Bland–Altman plot of the PPGAIxao.

 Parameter Method Age Questionaire BMI Calculated Waist circumference Measuring tape S-Chol Cobas-600-c501 commercial kit S-HDL-Chol Cobas-600-c501 commercial kit S-LDL-Chol Cobas-600-c501 commercial kit fS-Trigl Cobas-600-c501 commercial kit DBPbrach Arteriograph measured SPBbrach Arteriograph measured SPBao Arteriograph calculated PPao Arteriograph calculated PWVao Arteriograph calculated "Gold standard" parameter AIxao Arteriograph calculated PPGAIxao Proposed method in this study

Table 1: Measured and calculated parameters and methods used.

 All (n=54) Group-1 (n=20) Group-2 (n=25) Group-3 (n=9) Pa Pb Pc Age range (years) 21-37 40-53 56-73 Anthropometric parameters BMI (kg/m2) 24.8 ± 3.3 23.5 ± 3.2 25.9 ± 2 24.9 ± 2 0.021 0.43 0.23 Waist (cm) 89 ± 9 86 ± 9 92 ± 10 90 ± 4 0.34 0.42 0.15 Laboratory parameters S-Chol (mmol/L) 5.4 ± 1.0 4.9 ± 0.9 5.6 ± 1.1 5.8 ± 0.7 <0.01 0.72 <0.01 S-HDL-Chol (mmol/L) 1.68 ± 0.6 1.7 ± 0.7 1.8 ± 0.6 1.4 ± 0.5 0.46 0.15 0.42 S-LDL-Chol (mmol/L) 3.3 ± 0.9 2.9 ± 0.9 3.5 ± 0.9 3.9 ± 0.6 <0.01 0.27 <0.01 fS-Trigl (mmol/L) 1.20 ± 0.6 1.05 ± 0.4 1.16 ± 0.6 1.67 ± 1.0 0.46 0.07 0.02 Peripheral arterial blood pressure measurements DBPbrach (mmHg) 80 80 86 80 <0.01 0.263 0.501 (78 - 90) (74 - 80) (80 - 91) (70 - 90) SPBbrach (mmHg) 122 118 130 128 0.013 0.759 0.011 (114 - 131) (110 - 122) (117 - 135) (123 - 136)

a Comparison between group-1 and group-2.

b Comparison between group-2 and group-3.

c Comparison between group-1 and group-3.

Table 2: Characteristics of study subjects.

 All (n=54) Group-1 (n=20) Group-2 (n=25) Group-3 (n=9) Pa Pb Pc Age range (years) 21 – 37 40 – 53 56 – 73 Aortic stiffness parameters SBPao (mmHg) 113 111 126 144 <0.05 0.163 <0.05 (105 - 136) (105 - 115) (104 - 139) (105 - 177) PPao (mmHg) 39 37 44 54 <0.05 0.231 <0.05 (35 - 50) (33 - 44) (37 - 54) (38 - 77) AIxao (%) 19 13 24 36 <0.01 0.060 <0.05 (12 - 35) (8 - 18) (13 - 36) (31 - 48) PWVao (m/s) 7.1 6.8 7.0 9.8 <0.05 <0.05 <0.01 (6.6 - 8.5) (6.3 - 7.3) (6.6 - 8.6) (8.3 - 12.1) PPGAIxao (%) 15 8 17 22 <0.05 0.081 <0.01 (7 - 22) (-2 - 16) (7 - 26) (20 - 31)

a Comparison between group-1 and group-2.

b Comparison between group-2 and group-3.

c Comparison between group-1 and group-3.

Table 3: Arterial stiffness related parameters in the study subjects.

 SCORE n AIxao (%) PPGAIxao (%) Low Risk <1% 39 19 14 Moderate Risk ≥1% and <5% 13 27 20 High Risk ≥5% and <10% 2 48 33

Table 4: Arterial stiffness related parameters in the study subjects according to SCORE.

Citation: Pikta M, Viigimaa M, Pilt K, Kööts K, Meigas K (2020) Association between Optical Signal Derived Aortic Augmentation Index and Cardiovascular Risk Factors in Healthy Volunteers. Rep Glob Health Res 3: 119. DOI: 10.29011/RGHR-119.100019