Journal of Agronomy and Agricultural Aspects (ISSN: 2574-2914)

research article

Total and Individual Glucosinolates of Newly Bred Open Pollinating Genotypes of Broccoli (Brassica oleraceaconvar. botrytis var. italica) Grown Organically: Effect of Genotype and Growing Season

Samira Sahamishirazi1*,Jens Moehring2, Sabine Zikeli1, Michael Fleck3, Wilhelm Claupein1, Simone Graeff-Hoenninger1

1Department of Agronomy, Institute of Crop Science, University of Hohenheim, Stuttgart, Baden-Wuerttemberg, Germany

2Department of Biostatistics, Institute of Crop Science, University of Hohenheim, Stuttgart, Baden-Wuerttemberg, Germany

3Kultursaat e.V. Breeding Association, Echzell, Hesse, Germany

*Corresponding author: Samira Sahamishirazi,Department of Agronomy, Institute of Crop Science, University of Hohenheim, 70599 Stuttgart, Baden-Wuerttemberg, Germany. Tel: +4917620750712; Email:samira.sahami@gmail.com

Received Date: 12 December, 2017; Accepted Date: 08 January, 2018; Published Date: 15 January, 2018

Considering the demand for broccoli cultivars suitable for organic production and the prohibition of cultivating CMS-F1 hybrid cultivars under organic farming condition, current study evaluated glucosinolate content of eleven newly bred open pollinating genotypes of broccoli by comparison with F1 hybrid cultivars over two growing seasons. Effect of genotype, growing season and their interaction on glucosinolates was assessed as well. The results indicated the determination of six individual glucosinolates including glucoiberin, glucosinigrin, glucoraphanin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin. Glucoraphanin was the major glucosinolate with the largest share in total-glucosinolates (more than 70%) and significantly higher concentration in fall. Total-glucosinolates and glucoraphanin ranged from 3.46 to 3.60 µmol g-1  DW and 1.44 to 1.69 µmol g-1   DW, respectively. We observed significant reduction in concentration of glucoraphanin, glucoiberin, 4-methoxyglucobrassicin and neoglucobrassicin in all genotypes in spring compared to fall growing season as the result of growing season significant effect. In contrast, glucobrassicin content of open pollinating genotypes was mostly stable across growing seasons. The genotype × growing season interaction did not affect the concentration of glucosinigrin and total-glucosinolates.  Genetic factor affected the concentration of all glucosinolates significantly and resulted in differences in individual glucosinolates content of open pollinating genotypes and F1 hybrid cultivars. However, the level of total-glucosinolates of newly bred open pollinating genotypes was similar to F1 hybrid cultivars (3.46 - 3.60 µmol g-1   DW). A study on the agronomic performance of the open pollinating genotypes supplements the outcomes of this study and helps breeders and farmers to select the promising genotypes.

Keywords: Broccoli; Genotype; Glucosinolates; Growing season; Open pollinating; Organic


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