Current Trends in Clinical Toxicology

research article

Protective Effects of Vitamins E and C Against Crude Oil Induced Reproductive Toxicity in Rats

Saviour Udo Ufot1, Uduak Onofiok Luke2, Friday Effiong Uboh1*, Eyong Ubana Eyong1

1Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, Nigeria

2University of Uyo Teaching Hospital, University of Uyo, Nigeria

*Corresponding author: Friday Effiong Uboh, Department of Biochemistry, University of Calabar, Nigeria.

Tel: +2348037094449; Email: fridayuboh@yahoo.com; fridayuboh@unical.edu.ng

Received Date: 22 May, 2018; Accepted Date: 18 June, 2018; Published Date: 25 June, 2018

1.                   Abstract

This study determined some female serum sex hormones, litter size, birth weight and tail lengths of offspring’s delivered by rats orally exposed to crude oil and concomitantly administered vitamins E and C, respectively; using one hundred and two (102) albino Wistar rats (24 males and 78 females), weighing 160 to l80g. The rats were respectively distributed into six groups, with seventeen rats (four males and thirteen females) in each group. Groups 1, 2, 3, 4, 5 and 6 animals were given distilled water, 600 IU/kg of vitamin C only, 400 IU/kg of vitamin E only, 60mg/kg bow of crude oil only, 60mg/kg bow of crude oil with concomitant administration of 400 IU/kg of vitamin E, and 60mg/kg bow of crude oil with concomitant administration of 600 IU/kg of vitamin C orally for 30 days, respectively. After 30 days, five female rats from each group were sacrificed and blood serum collected for sex hormonal assay. The remaining rats were mated (one male to two females) until pregnancy was observed, after which they were withdrawn from experimental treatments. On delivery, the litter size for each group was recorded, total body weight and tail length of each litter were measured at birth and at weekly intervals for six weeks. The results of this study showed that crude oil produced a significant (p<0.05) decrease in serum estradiol and progesterone levels in female rats, compared to control; and that vitamins C and E attenuated this effect by producing a significant increase in the hormonal levels of rats exposed to crude oil. Also, a significant (p<0.05) decrease in litter size was recorded for rats given crude oil only, compared to the control. The mean total body weight of the litters delivered by rats treated with crude oil, at birth was insignificantly lower, compared to those in control. However, the weekly mean total body weight of the litters from the group treated with crude oil, for six weeks, was significantly (p<0.05) lower, compared to the litters from rats in the control group. Also, the mean tail length of the litters delivered by rats treated with crude oil, at birth and weekly for six weeks were insignificantly (p>0.05) lower, compared respectively, to the control group at corresponding intervals. The results also indicated that administration of vitamins C and E, respectively, to rats exposed to crude oil produced a significant reversal of the crude oil-induced decrease in litter size, birth weight and litter tail lengths in rats. It may therefore be concluded that vitamins C and E may be used to ameliorate crude oil-induced reproductive toxicity (decreased serum estradiol and progesterone, litter size, birth weights and tail length) in rats.

2.                   Keywords: Crude Oil; Litter Size; Litter Weight, Litter Tail Length, Serum Sex Hormones.